Endophytic colonization by the fungus Fusarium oxysporum can result in increased host resistance to pests and diseases, and greater biomass production. However, few studies have assessed the field performance\n of this fungus for biological control of pests and diseases in banana. Further to greenhouse assessment, studies were carried out to evaluate the performance of F. oxysporum strains against plant-parasitic\n nematodes on banana (Musa sp., cv. Giant Cavendish and cv. Grand Nain) in the field using tissue-cultured plants. Plants were inoculated separately with one of three strains (V5W2, Eny 7.11o and Emb 2.4o)\n before being inoculated with Pratylenchus goodeyi and Helicotylenchus multicinctus in an on-station trial and in an on-farm trial planted in a field naturally infested with the same nematodes. All three endophytic\n strains significantly suppressed P. goodeyi and H. multicinctus densities and damage in the field. On-station, nematode population densities were reduced by >45% in endophyte-inoculated plants compared to non-inoculated plants, while percentage root necrosis was reduced by >20%. Similarly, on-farm, nematode damage to roots and densities were also significantly lower in endophyte-inoculated plants compared with control plants. Significantly improved yields were observed for plants inoculated with endophytes when compared to the control plants, with inoculation with strains Emb 2.4o and V5W2 resulting in up to 35% and 36% increased banana yields, respectively, for the on-station trial. For the on-farm trial, up to 20% increase in yields were observed for strain Eny 7.11o compared to control plants.\n This study provides the first report from the field in Africa on the reduction of nematode populations and damage, and the increase in banana production by fungal endophytes. The study shows that endophytes have potential to enhance yields of tissue-cultured banana plants and protect them against pests.