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Abstract

A simple and inexpensive procedure that uses freshly emerged soybean (Glycine max (L.) Merril) cotyledons is developed for mass-rearing of bean fly, Ophiomyia phaseoli (Tryon). Bean fly adults lay eggs in the soybean cotyledons and the larvae feed and pupate in that plant part. Larvae and pupae at any stage of development can be removed and used for studies or the pupae can be maintained until adult emergence. The insect raised in this manner retains its ability to infest soybean. We have raised up to 2,000 flies per day by this method with the input of one laborer. [AS]

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