The success and sustainability of chili pepper breeding depends upon diversity in genetic diversity resources. The genetic diversity can be increased through the introduction of material from other countries. The present study aimed to measure the contribution of introduced lines to enrich diversity of IVEGRI’s breeding program based on morphological characterization. The experiment was carried out at IVEGRI research station from July to December 2020, and arranged with two replications in a randomized block design. The treatments involved 60 genetic materials, 30 of which were derived from Indonesia and other 30 lines were introduced from World Vegetable Center. Morphological traits were measured following the guidelines for testing the distinctness, uniformity, and stability from Indonesian Plant Variety Protection Center, Ministry of Agriculture. Genetic diversity analysis was conducted using the Euclidean dissimilarity coefficient. The results showed an increase of 88.2% in genetic distance after introduction. The unique morphological characters, such as shortened internodes, hairiness of nodes, leaf shapes, secondary color of corolla, fruit color before maturity, fruit color at maturity, and fruit attitude, were observed from the introduced chili pepper lines reflecting that those could be beneficial for future improvement of chili pepper breeding program.