Evaluation of genetic diversity in Vigna radiata (L.) using protein profiling and molecular marker (RFLP)

Evaluation of genetic diversity in Vigna radiata (L.) using protein profiling and molecular marker (RFLP)

2015

E12945

Seed storage protein profiling through SDS-PAGE and DNA Restriction Fragment Length Polymorphism (RFLPs) were surveyed in 5 accessions of mungbean (Vigna radiata L.) sampled from NBPGR Thrissur, Kerala, India, to evaluate genetic diversity. The protein profile showed a low level of polymorphism (26.66%). The similarity index calculated ranged from (42.30-50%). The dendrogram constructed revealed two clusters. Cluster 1st comprises two accessions IC 251432 and IC 251433. Cluster 2nd comprises 3 cultivars viz., IC 251431, IC 251430 and IC 251434. Cluster 2nd also revealed a 100% homology between IC 251431 and IC 251430 accessions. The RFLP analysis was carried out by single restriction enzyme EcoRI. The RFLP profile revealed a high level of polymorphism (90.90%). A total of 11 clear bands were scored. The only band with fragment size 630 kb was monomorphic while, the rest were polymorphic. Similarity index ranged from (42.82-11.11%). The UPGMA analysis divided the accessions into 2 clusters viz., cluster 1st comprising 4 accessions (IC 251431, IC 251432, IC 251433 and IC 251430). Cluster 2nd includes only one accession (IC 251434) occupying a distinct position in constructed dendrogram.

9(4):238-246, INTERNATIONAL JOURNAL OF PLANT BREEDING AND GENETICS

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