First report and molecular characterization of an isolate of Papaya ringspot virus (PRSV-W) detected in pumpkin in Cuba
2015
E12341
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Title
First report and molecular characterization of an isolate of Papaya ringspot virus (PRSV-W) detected in pumpkin in Cuba
Publication Date
2015
Call Number
E12341
Summary
In this work, a virus isolate collected from pumpkin plants (Cucurbita pepo L.), showing severe symptoms of mosaic and leaf deformation, grown in Cuba, was analyzed using indicator plants, electron microscopy, and phylogenetic analysis. Plants of pumpkin, cv. Caserta, inoculated with this virus isolate showed mosaic, leaf distortion and blistering symptoms, whereas papaya plants were immune and did not show any symptoms. A transmission electron microscopic examination of leaf dip preparations made from infected pumpkin leaves revealed the presence of elongated and flexuous particles, approximately 780-800x12 nm in size. Genomic fragments containing the coat protein (CP) and HC-Pro genes, amplified by specific primers for Papaya ringspot virus, W strain (PRSV-W), showed amino acid identities of both genes higher than 94% when compared to other PRSV-W isolates from America. In the phylogenetic tree, this virus isolate has grouped with other virus isolates from America, Australia, and India and was more distant from the Asian isolates. Taken together, the analyses allow the conclusion that this virus isolate is a W strain of PRSV, detected for the first time in Cuba.
Journal Citation
31(4):1133-1142, BIOSCIENCE JOURNAL
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles