Preliminary phytochemical screening and HPTLC fingerprinting profile of leaf extracts of Moringa oleifera and Phyllanthus emblica.

Preliminary phytochemical screening and HPTLC fingerprinting profile of leaf extracts of Moringa oleifera and Phyllanthus emblica.

2015

E11360

Objective: To establish the physical constants and fingerprint profile of Phyllanthus emblica and Moringa oleifera using high performance thin layer chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done, physical constants were evaluated and HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5 applicator, TLC scanner 3, Reprostar 3 and WIN CATS-1.4.3 software were used. Results: Preliminary phytochemical screening of the extract showed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, terpenoids, phenolic compounds, proteins, reducing sugars, fats and oils. HPTLC finger printing of petroleum ether extract of Moringa oleifera leaf powder under white light reveal presence of eight components 0.24, 0.29, 0.34, 0.54, 0.76, 0.83, 0.93, 0.99. Component number 8 at Rf 0.99 showed maximum concentration. Petroleum ether extracts of Phyllanthus emblica leaf powder showed seven peaks with Rf values in the range with their Rf value - 0.26 0.34 0.55, 0.77 0.80, 0.95. Component number 6 at 0.95 Rf value showed maximum concentration. Conclusions: It can be concluded that HPTLC fingerprint analysis of Petroleum ether extract of leaf powder extracts of Phyllanthus emblica (PELPE) and Moringa oleifera (PELMO) can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant populations.

2(2):32-40, INTERNATIONAL RESEARCH JOURNAL OF PHARMACEUTICAL AND BIOSCIENCES

Research > Published Articles