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Title
Protective roles of brassinolide on tomato seedlings under drought stress
Author
Behnamnia, M.
Publication Date
2015
Call Number
E11333
Summary
Tomato (Lycopersicum esculentum L.) was used to study the role of 24-epibrassinolide (24-EBL) in protection from drought stress. In this experiment, when the third leaf of tomato plants appeared, 24-epibrassinolide was sprayed at 0.01 and 1 micro M concentrations for 3 days alternately. Then three levels of drought stress (control, 3 and 5 days withholding water) were applied. Thereafter, Analysis was conducted on the contents of leaf dry and fresh weight, water content, chlorophylls, reduced and total carbohydrates, ethylene and the leakage of electrolyte. Also the activities of lypoxygenase (LOX) and superoxide dismutase (SOD) and SOD isozymes expression levels in leaves. The result showed that Reduction in weight, chlorophylls, reduced and total carbohydrates content in drought condition probably due to the effect of drought on chlorophyll synthesis and degradation and in turn on photosynthesis. Also drought-induced increase in lypoxygenase activity caused an increase in ion leakage. However, alleviation in ion leakage, lypoxygenase activity and ethylene production in plants treated with concentration of 24-EBL under drought stress was observed. This could be because of the positive effect BR on stability membrane. Under the drought treatment, application of BR significantly increased leaf weight and water, the contents of chlorophyll and carbohydrates, and the activity of SOD and the expression of SOD isozymes in the leaves. Therefore, probably our results showed that 24-EBL could decline oxidative damage caused by drought stress and that BR plays an important role in protection of tomato plants from drought stress by enhancing the activities or expression level of protective enzymes in the leaves.
Journal Citation
8(3):455-462, INTERNATIONAL JOURNAL OF AGRICULTURE AND CROP SCIENCES
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles