Validation of molecular markers linked to alleles controlling growth habit in common bean (Phaseolus vulgaris L.)

Validation of molecular markers linked to alleles controlling growth habit in common bean (Phaseolus vulgaris L.)

2014

E10473

This work had objectives to determine the inheritance growth habit of the Andean cultivar Jalo-UEM using the F2 populations from the Jalo-UEM x Cornell 49-242 and Jalo-UEM x Michelite crosses; and, to investigate the utility of Bmd45-AIA molecular marker for identifying plants having FinFin and/or finfin alleles in these segregating populations. Phenotypic and molecular analyses were conducted in two segregating populations derived from crosses between a line displaying a determinate growth (Jalo-UEM) and two cultivars with an indeterminate growth (Cornell 49-242 and Michelite) habit. A total of 21 molecular markers were tested with different amplification patterns in parents and populations. The marker that showed polymorphism was genotyped in F2 populations of crosses. In the F2 populations of the Jalo-UEM x Cornell 49-242 and Jalo-UEM x Michelite crosses, the phenotypic ratio of the two growth habits was 3Fin: 1fin, with p=0.34 and p=0.62, respectively, for both crosses. This result demonstrates that the inheritance of the indeterminate growth habit was conferred by a single dominant gene, Fin. Molecular analysis using the microsatellite marker Bmd45-AIA revealed co-dominant segregation for both study populations, with fragment sizes of 207 and 245 bp linked to the Fin (indeterminate) and fin (determinate) alleles, respectively. The segregation ratio of this marker was 1Fin/Fin: 2Fin/fin: 1fin/fin in the F2 populations from the Jalo-UEM x Cornell 49-242 (p=0.56) and Jalo-UEM x Michelite (p=0.42) crosses. This study further establishes the relevance of the Bmd45-AIA marker for marker-assisted selection of common bean plants architecture and should allow common bean breeders to identify plants with different growth habit.

8(11):1503-1509, AUSTRALIAN JOURNAL OF CROP SCIENCE

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