Deregulation of mitosis progression and cytotoxic effect triggered in Allium cepa L. roots by Rubus sancatus schreber extract

Deregulation of mitosis progression and cytotoxic effect triggered in Allium cepa L. roots by Rubus sancatus schreber extract

2014

E10273

Background and Aims: Medicinal plant extracts have been traditionally used in curing several diseases in folk medicine but several of them proved to cause cellular changes in many vital systems. Family Rosaceae demonstrated different biological activities in several previous investigations. The present study aims to assess the\n cytological and cellular effect of different concentrations of Rubus sanctus Schreber aqueous alcoholic extract (RsAAE) on Allium cepa L. root meristems using different techniques. Material and Methods: Cytological and cellular effect of different concentrations (1, 5& 10 mg mL-1) of RsAAE for different duration of administration (3, 6& 12h) on A. cepa L. root cells were studied using mitotic, ultrastructural and biochemical analyses. Result: The\n administration of different concentrations of RsAAE for different periods of exposure exhibited suppression of the mitotic and cell cycle progression on A. cepa root meristems. Several mitotic abnormalities were recorded. RsAAE enhanced deleterious changes on the cellular organelles of A. cepa root meristems leading to autolysis of the cells. Gene expression was changed as 10 de novo bands were produced as well as several bands were disappeared, these alterations were associated with a drastic inhibition of protein content. Discussion: The present study declared the prevalence mitostatic and cytotoxic effect of RsAAE on A. cepa root meristems. In spite of R. sanctus has valuable effects as a medicinal herb, it can enhance severe complications and destruction on the normal cells when it is used indecorously.

11(11):1047-1058, LIFE SCIENCE JOURNAL

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