Effect of Allium cepa L. on lipopolysaccharide-stimulated osteoclast precursor cell viability, count, and morphology using 4,6-diamidino-2-phenylindole-staining

Effect of Allium cepa L. on lipopolysaccharide-stimulated osteoclast precursor cell viability, count, and morphology using 4,6-diamidino-2-phenylindole-staining

2014

E09584

Allium cepa L. is known to possess numerous pharmacological properties.Our aimwas to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based assays. Osteoclast precursor cells (RAW264.7) at 37C/5% CO2. Resazurin reduction and total protein content assayswere used to detect cell viability.AcE did not affect cell viability. Resazurin reduction assay showed that AcE, at up to 1000 ug/mL, did not significantly affect cell viability and cellular protein levels. Additionally a caspase 3/7 luminescence assay was used to disclose apoptosis and there was no difference in apoptotic activity between tested groups and control group. Fluorescence images stained by DAPI showed no alteration on the morphology and cell counts of LPS-stimulated osteoclast precursor cells with the use of AcE in all tested concentrations when compared to control. These findings suggest that Allium cepa L. extract could be used for in vitro studies on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells.

2014:535789, INTERNATIONAL JOURNAL OF CELL BIOLOGY

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