An SSR-based linkage map of yardlong bean (Vigna unguiculata (L.) Walp. subsp, unguiculata Sesquipedalis Group) and QTL analysis of pod length
2012
E09023
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Title
An SSR-based linkage map of yardlong bean (Vigna unguiculata (L.) Walp. subsp, unguiculata Sesquipedalis Group) and QTL analysis of pod length
Author
Kongjaimun, A.
Kaga, A.
Tomooka, N.
Somta, P.
Shimlzu, T.
Shu, Y.
Isemura, T.
Vaughan, D.A.
Srinives, P.
Kaga, A.
Tomooka, N.
Somta, P.
Shimlzu, T.
Shu, Y.
Isemura, T.
Vaughan, D.A.
Srinives, P.
Publication Date
2012
Call Number
E09023
Summary
Yardlong bean (Vigna unguiculata (L.) Walp. slbsp. ung,uiculara Sesquipedalis Group) (2n = 2x = 22) is one of the most important vegetable legumes of Asia. The objectives of this study were to develop a genetic linkage map of yardlong bean using SSR makers from related Vigna species and to identify QTLs for pod length. The map was constructed from 226 simple sequence repeat (SSR) markers from cowpea (Vigna unguiculata (L.) Walp. subsp. unguiculata Unguiculata Group), azuki bean (Vig,na angularis (Willd.) Ohwi & Ohashi), and mungbean (Vig,na radiata (L.) Wilczek) in a BCrFr (JPS1610 x TVnu457) x JPSl610) population derived from the cross between yardlong bean accession JP8l6l0 and wild cowpea (Vigna unguiculata subsp. unguiculata var. spontanea) accession TVnu457. The markers were clustered into I I linkage groups (LGs) spanning 852.4 cM in total length with a mean distance between adjacent markers of 3.96 cM. All markers on LG11 showed segregation distortion towards the homozygous yardlong bean JP816l0 genotype. The markers on LGll were also distorted in the rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi) map, suggesting the presence of common segregation distortion factors in Vigna species on this LG. One major and six minor QTLs were identified for pod\n length variation berween yardlong bean and wild cowpea. Using flanking markers, six of the seven QTLs were confirmed in an F2 population of JPSl610 x TVnu457. The molecular linkage map developed and markers linked to pod length QTLs would be potentially useful for yardlong bean and cowpea breeding.
Journal Citation
55:81-92, GENOME
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles