Drug resistance and antifungal activity of garlic and neem extracts on Candida SPP. isolates from HIV-infected patients

Drug resistance and antifungal activity of garlic and neem extracts on Candida SPP. isolates from HIV-infected patients

2013

E06810

The objective of the present study was to investigate drug resistance and antifungal activity of garlic and neem extract on candida species, which were isolated from HIV-infected patients. A total of 150 candida isolates from HIV-infected patients, which were previously isolated and identified were included in this study. In vitro antifungal susceptibility to fluconazole, itraconazole and amphotericin B was evaluated. Antifungal potency of garlic and neem leaves extract against all the isolates was tested. Among all isolates, 24% (36) were resistant to fluconazole, 28.7% (43) to itraconazole and 16.7% (25) to amphotericin B. The isolates demonstrated high fluconazole MICs, in which 8% presented values of 128 μg/ml followed by itraconazole in which 10.7% presented values of 4 μg/ml. All isolates demonstrated low garlic extract’s MIC50 in which 4% (6) isolates presented values of 0.097 mg/ml and 86.7% (130) presented values of 0.195 mg/ml. When treated with neem extract, 93.3% (140) presented MIC50 value\n of 0.390 mg/ml. The isolates were completely inhibited by garlic and neem leaves extract with MFC value of 0.781 mg/ml and 1.562 mg/ml respectively. The present study has thus confirmed the antifungal potency of garlic and neem against drug resistant candida isolates from HIV-infected patients. The observed zones of growth inhibition and MICs showed that the isolates exhibited susceptibility. This indicates garlic and neem have a broad spectrum of antifungal activity. The MFCs of garlic extract reported in this study are in consonance with previous anticandidal studies on pathogenic and emerging drug resistant Candida species.

4(2):1426-1433, INTERNATIONAL JOURNAL OF ADVANCES IN PHARMACEUTICAL RESEARCH

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