Molecular diversity among selected Momordica cochinchinensis (Lour.) spreng accessions using rapd markers

Molecular diversity among selected Momordica cochinchinensis (Lour.) spreng accessions using rapd markers

2012

E06661

Spiny bitter gourd or gac fruit (Momordica cochinchinensis (Lour.) Spreng) is a minor cucurbitaceous vegetable crop in spite of having considerable nutritional and medicinal properties. Although there have been some reports on its genetic diversity based on molecular markers, no work has been conducted to estimate genetic diversity in this crop. The aim of this research was to study genetic diversity and genetic relatedness of spiny bitter gourd collected in Thailand and Vietnam using RAPD markers. In the present study, 25 accessions of M. cochinchinensis including from different parts of Thailand and Vietnam were analyzed for diversity study at molecular levels. Genomic DNA was extracted from young healthy leaves following the procedure of DNA Trap I (DNA Technology Laboratory, Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom). Pair-wise comparison of genotypes was calculated as per the procedure of Jaccard. Dendrogram was constructed using the unweighted pair group method with arithmetic averages (UPGMA) and the computation for multivariate analysis was done using the computer program NTSYS-pc Version 2.0. Among 36 random decamer primers screened, 11 were polymorphic and informative enough to analyze these genotypes. A total of 176 markers generated of which 175 (99.43%) were polymorphic and the number of bands per primer was 16.00 out of them 15.91 were polymorphic. The coefficients of genetic similarity within spiny bitter gourd accessions were from 0.63 to 0.90, suggesting a wide genetic base for the genotypes. The spiny bitter gourd accessions were grouped into eight major clusters. This study could also clearly identify male and female genotypes using molecular markers, and this information is very useful for crop breeding and crop production.

44(2):406-417, SABRAO JOURNAL OF BREEDING AND GENETICS

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