Details
Title
Study on cryopreservation for shoot tip of sweet potato
Author
Yu, P.C.
Hung, C.H.
Lo, S.F.
Hung, C.H.
Lo, S.F.
Publication Date
2012
Call Number
A:PS
Summary
This is the first report describing the use of encapsulation-vitrification or aluminum cryoplate technology for cryopreservation of sweet potato and then to regenerate new plants in Taiwan. The shoot tips of sweet potato [Ipomoea batatas (L.) Lam.] cultivars Tainung Nos. 57, 66, 71 and 73 were adopted for the experiment. The results showed the survival rate of the shoot tips cluld increase to 60-100% by pre-culture with MS (Murashige and Skoog) basic salted medium with 0.3 M sucrose and 0.9% Difco agar for 4 d after treatment of loading solution (LS) and plant vitrification solution 2 (PCS2). In the method of encapsulation-vitrification, the shoot tips of four sweet potato cultivars were osmoprotected with LS for 300 min and then dehydrated with PVS2 for 60 min before immersing into liquid nitrogen. The regeneration rate reached 3.3% for all these four cultivars. By replacing PVS2 solution with plant vitrification solution 3 (PVS3) for dehydration and osmoprotection before immersion into liquid nitrogen, both Tainung No. 66 and No.71 reached 3.3% regeneration rate. With a.uminium cryo-plate technology for cryopreservation, the regeneration rate of Tainung No.66 reached 3.3% when treated with both LS and PVS2 solutions for 30 min each. Tainung No.71 had the same rate when treated with 30 min LS and 15 min PVS2. By comparing the results obtained from these two technologies, the cryopreservation using aluminum cryo-plate reduced 45% of processing time and 70% of chemical used. Accordingly, aluminum cryo-plate technology seems a better method for cryopreservation of shoot tips of sweet potato.
Journal Citation
9(4):209-219, CROP, ENVIRONMENT & BIOINFORMATICS
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles