A set of allele-specific markers linked to L locus resistant to Tobamovirus in Capsicum spp.

A set of allele-specific markers linked to L locus resistant to Tobamovirus in Capsicum spp.

2012

A:PS

The resistance to Tobamovirus in Capsicum spp. has been known to be controlled by five different\n alleles (L0, L1, L2, L3, and L4) of L locus on the telomere of long arm of pepper c omosome 11. To eve op a set of molecular markers differentiating all the alleles of L locus, we used five pepper differential hosts including Capsicum annuum Early California Wonder (ECW, L0L0), C. annuum Tisana (L1L1), C. annuum Criollo de Morelos 334 (CM334, L2L2), Capsicum chinense PI 159236 (L3L3), and Capsicum chacoense PI 260429 (L4L4). Developing a series of CAPS or SCAR markers specifically linked to the alleles was allowed by the sequence comparison of PCR amplicons of the L3-linked markers (189D23M, A339, and 253AIR) and BAC sequences (FJ597539 and FJ597541) in the pepper differentials. Genotypes deduced by these markers in 48 out of 53 F1 hybrids of commercial pepper varieties were consistent with their phenotypes by bioassay using Tobamovirns pathotypes (P0, P1, and P1,2). Consequently, these markers can be useful to differentiate L alleles and for breeding Tobamovirus resistance in pepper with marker-assisted selection.

30(3):286-293, KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY

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