Record Details

Title:
Chitinase from soybean seeds: purification and some properties of the enzyme system
Publication date:
1984
Summary:
Chitinase was isolated and purified from soybean seed extract for the first time by ammonium sulfate precipitation followed by chromatography on ground chitin. Five protein peaks containing chitinase activity were eluted, with three peaks containing chitobiase activity as well. Purification was up to 258-fold. The procedure is simple, inexpensive and yielded chitinase of higher specific activity than two out of three commerical enzymes tested. The average molecular weight of the chitinase was 31600 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Preliminary results indicate the enzyme acts as an endochitinase and that isoenzymes may be present. The enzyme exhibited a relatively low pH optimum in the range of 3.3-4.0. Activity on regenerated chitin was considerably greater than on more crystalline native substrates. [AS/SS]
Call number:
A:PS
Journal citation:
Contact information:
harvest@worldveg.org

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 Record created 1985-06-03, last modified 2019-03-04


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