Inheritance analysis of fertility restoration genes (Rf) in a male sterile system of eggplant using cytoplasm of Solanum grandifolium

Inheritance analysis of fertility restoration genes (Rf) in a male sterile system of eggplant using cytoplasm of Solanum grandifolium

2012

E05087

Analysis of pollen and seed fertility of the test cross progenies was performed to assess the inheritance and relevancy of male fertility restoration ability of Solanum grandifolium, an induced male-sterile eggplant. In this study, 'Taibyo VF' (S. grandifolium x S. melongena) was continuously backcrossed to recurrent pollen parent S. melongena, to develop a cytoplasmic substitution line of eggplant. All the examined BC1 plants were male fertile while the BC2 plants segregated into male fertile and sterile types. The BC3, BC4, BC5 and BC6 progenies, obtained from the male fertile seed parents, were also segregated into the above mentioned types, whereas backcross progenies obtained from male-sterile seed parents were all male-sterile without any segregation. Backcross and selfed progenies obtained from male fertile plants were fitted well to either 1:1 or 3:1 and 3:1 or 15:1 ratios, respectively. The progenies obtained from crossing between male fertile and sterile backcross progenies were fitted to 3:1 ratio. The segregation patterns indicated that two independent dominant fertility restorer (Rf) genes control the pollen formation of the S. melongena with the cytoplasm of S. grandifolium. It is suggested that progenies containing one or both of dominant Rf loci will allow pollen formation while recessive Rf loci will result the non pollen-forming plants. The pollen stainability was about 50% and 85%, when the male fertile plants pollinated with a male donor containing S. melongena and S. grandifolium cytoplasms, respectively. Knowledge of male fertility restoration inheritance of this Cytoplasmic male sterility (CMS) is useful to develop and utilize the restorer lines in the breeding of eggplant.

6(3):476-479, AUSTRALIAN JOURNAL OF CROP SCIENCE

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