Genetic analysis and mapping of gl-2 gene in cucumber (Cucumis sativus L.)

Genetic analysis and mapping of gl-2 gene in cucumber (Cucumis sativus L.)

2011

A:PS

Genetic analysis and gene mapping were carried out on gl-2 gene in cucumber (Cucumis sativus L. ) using 911 OGt ( with trichomes) and NCG-042 (glabrous mutant) as experimental materials. The genetic analysis showed having trichomes or not is determined by a single nuclear gene, and the trait of having trichomes (Gl-2) is dominant to the glabrous (gl-2) in cucumber. Bulked segregant analysis (BSA) and simple sequence repeat (SSR) techonology were employed to mapping gl-2 gene of cucmber in F2 population. gl-2 gene was mapped to a linkage group with 11 SSR makers, corresponding to chromosome 2 of cucumber. The flanking markers SSR1O522 and SSR13275 1 were linked to the gl-2 gene with genetic distances of 0.6 and 3.8 cM, respectively. The veracity of SSR1O522 and SSR13275l was tested using BCjP2 population, and the accuracy rate for the two markers was 94.4% and 91.6%.

v.38(9):1685-1692, ACTA HORTICULTURAE SINICA

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