Factors affecting embryogenesis in isolated microspore culture of broccoli (Brassica oleracea var. italica)

Factors affecting embryogenesis in isolated microspore culture of broccoli (Brassica oleracea var. italica)

2010

A:PS

Thirteen varieties of F1 hybrid broccoli were selected as materials to investigate the factors impacting mi­crospore embryogenesis in isolated microspore culture. The results showed that genotype was one of the key factors in embry­oid induction of broccoli, Embryoid was induced in eight genotypes, the induction ratio being 61.5% . The optimum time period for the microspore embryogenesis was from uninucleate stage to binucleate stage. It was favorable for embryo induc­tion to add appropriate amount of sucrose, activated carbon, 6- BA, AgNO3 and 2, 4- D into 1/2 NLN medium. The pretreat­ment at 4 degrees C for 24 h and the heat-shock treatment at 32.5 degreesC for 24 h were beneficial for the embryogenesis.

v.26(6):1319-1322, JIANGSU JOURNAL OF AGRICULTURAL SCIENCES

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