Analysis of bolting trait of Brassica campestris L. ssp. Chinensis var. utilis Tsen et Lee by ISSR and SRAP makers
2009
A:PS
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Title
Analysis of bolting trait of Brassica campestris L. ssp. Chinensis var. utilis Tsen et Lee by ISSR and SRAP makers
Publication Date
2009
Call Number
A:PS
Summary
The F2 population derived from a cross between CT7 ( early bolting) and T0601 (late bolting) was used to identify bolting trait using ISSR and SRAP makers by BSA analysis. 45 ISSR primers and 20 SRAP primers combinations were used to screen polymorphism in parents and DNA pools. PCR pattern in parents and DNA pools showed different fragment appeared in CT07 and early bolting pools by UBC834, UBC876, E13M4 and E5M7 primers. Bolting trait was linked to four makers and the closest marker to bolting gene was E13M4 and the distance between them was 16. 8 cM through F2 generation individuals identification. The four makers in the study could be used to maker assistant breeding.
Journal Citation
v.25(4):829-833, JIANGSU JOURNAL OF AGRICULTURAL SCIENCES
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