Development of gene-based markers for the Pun1 pungency gene in pepper (Capsicum spp.) for marker-assisted selection

Development of gene-based markers for the Pun1 pungency gene in pepper (Capsicum spp.) for marker-assisted selection

2009

A:PS

Pungency in fruits of pepper (Capsicum spp.) is due to the accumulation of capsaicinoids. Pun] is an important gene responsible for pungency. To provide information for marker-assisted selection (MAS) of pungency, we developed and evaluated five co dominant markers based on sequence of the Pun] gene. The usefulness of these gene-based markers for pungency in MAS was tested using 85 pungent and non-pungent lines and cultivars. Our results demonstrated that these markers can discriminate the pungent and non-pungent genotypes. Our results show that the five gene-based markers could be useful as a marker-assisted selection tool.

v.50(4):358-365, HORTICULTURE, ENVIRONMENT, AND BIOTECHNOLOGY

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