Resistance of tomato seedlings to early blight

Resistance of tomato seedlings to early blight

1969

A:PS

Abundant sporulation of Alternaria solani grown on reconstituted lima bean agar at 23 C was induced by scraping aerial mycelium from 1-week-old cultures, and then exposing the cultures (with the culture dish lids removed) on a laboratory bench to indirect sunlight, plus 200 ft-c of cool-white fluorescent light during the day, and to darkness at night. Tomato seedlings growing in 1800 cm2 flats were inoculated in the 3-leaf stage by atomizing onto the leaves 30 ml of suspension containing 20,000 spores/ml distilled water. The plants were incubated in a mist chamber at 21-24 C for 24 hr, and then placed on a bench in a greenhouse where the temperature was 24 + 3 C. Rapid development of target-spot lesions occurred in 1 week when plants were returned to the mist chamber for 16 hr nightly, and to the greenhouse bench daily.

v.59:443-446, PHYTOPATHOLOGY

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