Proteomic analysis of F2 generation of cucumber against the cucumber powdery mildew disease
2007
A:PS
Details
Title
Proteomic analysis of F2 generation of cucumber against the cucumber powdery mildew disease
Publication Date
2007
Call Number
A:PS
Summary
Proteomic approaches using 2-dimensional gel electrophoresis (2-DE) were adopted to identify the proteins from cucumber leaves differentially expressed in the F2 generation of cucumber. Approximately 500 proteins were reproducibly detected, including 39 specific proteins expressed in the F2 sensitive pool samples and 22 specific proteins expressed in the resistance pool samples; 35 proteins were 2-fold upper limit and 46 proteins were 2-fold lower limit. Analysis with MALDI-TOF-MASS showed that 9 important proteins associated with defensive reaction were identified in the F2 cucumber, a protein of disease resistance gene (SSP3110), 14-3-3 brain protein homologue (SSP1411), coproporphyrinogen-III oxidase [coproporphyrinogen oxidase] (SSP5513), carbonic anhydrase [carbonate dehydratase] (SSP5412), alpha-galactosidase-like protein (SSP5710), putative protein (SSP6002), 17.8 kDa class small heat shock protein (SSP0710), hypothetical protein (SSP3111), (S)-2-hydroxy-acid oxidase (EC 1.1.3.15)-cucurbit (SSP4610). All these proteins were probably parts of network of resistance or development-related proteins. This study gives new insights into cucumber powdery mildew (Sphaerotheca fuliginea) resistance response in cucumber leaves and demonstrates the power of the proteomic approach in plant biology studies.
Journal Citation
v.34(2):349-354, ACTA HORTICULTURAE SINICA
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles