Micropropagation of Capsicum frutescens L. using axillary shoot explants

Micropropagation of Capsicum frutescens L. using axillary shoot explants

2007

A:PS

A novel micropropagation protocol was established for Capsicum frutescens L. cv. 'Uchithi', a pungent chilli cultivar, through induction of axillary shoot proliferation of in vitro raised plantlets by decapitation and using the axillary shoots as explants for multiple shoot bud induction. About 2-6 axillary shoots were induced within 2 weeks when 4-week-old in vitro raised plantlets were decapitated. The axillary shoot-tip explants produced multiple shoot buds when cultured on Murashige and Skoog's (MS) medium containing 8.8-44.4 micro M 6-benzylaminopurine (BAP) or 9.3-46.7 micro M kinetin alone or 8.8-44.4 micro M BAP with 4.6 micro M kinetin or 5.7 and 28.5 micro M indole-3-acetic acid (IAA). Maximum number of shoots (5.6) were induced on medium containing 22.2 micro M BAP in combination with 4.65 micro M kinetin. The separated shoots rooted and elongated on medium containing 2.8 micro M IAA or 2.4-4.9 micro M indole-3-butyric acid (IBA). Rooted plantlets were successfully established in the soil. Efficient mass multiplication of this important food crop was achieved.

v.113(1):96-99, SCIENTIA HORTICULTURAE

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