Control of mycoflora of farmer's stored seeds of mungbean

Control of mycoflora of farmer's stored seeds of mungbean

2007

REP.MB-3155

The prevalence of fungi and bacteria associated with mung bean (Vigna radiata) seeds and their control were studied during January-November 2004. Twenty seed samples of mung bean were collected from two villages of Mymensingh district, Bangladesh. The germination percentage of all samples of mung bean remarkably varied. Hot water treatment at 53 degrees C for 15 min showed comparatively better performance than Vitavax 200 [carboxin+thiram] at 2.5 g kg-1 in the case of germination. Seed-borne fungi were recorded and detected by using blotter method. Four different fungal pathogens were identified, i.e. Aspergillus flavus, Aspergillus niger, Fusarium spp. (F. oxysporum, F. moniliforme [Gibberella moniliformis] and F. semitectum [F. pallidoroseum]) and Penicillium spp. Two groups of bacteria were identified on ungerminated seeds and emerging radicles. Yellow and creamy coloured bacterial colonies were found in seed surface during blotter incubation test. The liquid assay was better than the washing test and blotter method for detecting pathogenic bacteria. Xanthomonas campestris pv. vignicola [X. axonopodis pv. vignicola] and Pseudomonas syringae pv. syringae were identified. The efficacy of two seed treating methods, i.e. Vitavax-200 and hot water, were tested in the laboratory. Vitavax 200 seed treatment at 2.5 g kg-1 showed higher control of seed-borne fungi than hot water treatment. Hot water treatment at 55 degrees C for 15 min was also effective in reducing the seed-borne fungi and bacterial infections. Hot water treatment at 55 degrees C hampered the germination percentage of seeds. The hot water treatment at 53 degrees C for 15 min was effective for controlling the seed-borne mycoflora, next to Vitavax 200 at 2.5 g kg-1.

v.6(1):115-121, ASIAN JOURNAL OF PLANT SCIENCES

Research > Published Articles