Estimation of nuclear DNA content of plants by flow cytometry

Estimation of nuclear DNA content of plants by flow cytometry

1991

E00241

A rapid and simple protocol for estimation of nuclear DNA content of plants is described. Suspensions of intact nuclei are prepared either by chopping plant tissues or lysing protoplasts in a MgSO 4 buffer, mixed with DNA standards, and stained with propidium iodide in a solution containing DNAase-free RNAase. Fluorescence intensities of the stained nuclei are measured by a flow cytometer. Values for nuclear DNA content are estimated by comparing fluorescence intensities of the nuclei of the test population with those of appropriate internal DNA standards. The same procedure can also be used for rapid determination of ploidy in plant tissues.

v.9(3):229-233, PLANT MOLECULAR BIOLOGY REPORTER

Research > Published Articles