Tobamoviruses of green peppers growing on hydroponic systems

Tobamoviruses of green peppers growing on hydroponic systems

2004

A:PS

Incidence of tobamovirus diseases was 100% at late growth stage of green pepper on hydroponic systems in plastic lumse. Infection frequency of the diseases showed 34% of Pepper mild mottle virus (PMMoV), 41.5% of Tobacco mild green mottle virus (TMGMV), and 24.5 % of the co-infected viruses. The two viruses specifically reacted in DAS- ELISA prepared with each polyclonal antibody. A total of 77 pure tobamovirus isolates obtained from the crops was tested for pathotype determination. The isolation frequency of tobamovirus pathotype P0 and P1,2 was 61 % and 39%, respectively. All TMGMV isolates belonged to the pathotype P0. In restriction enzyme analysis of the cDNAs synthesized with coat protein gene ofPMMoV pathotype P0 and P1,2, the former had two TaqI sites but the later had one.

v.10(3):194-197, RESEARCH IN PLANT DISEASE

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