DNA markers linked to pepper mild mottle virus (PMMoV) resistant locus (L4) in Capsicum
2003
A:PS
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Title
DNA markers linked to pepper mild mottle virus (PMMoV) resistant locus (L4) in Capsicum
Publication Date
2003
Call Number
A:PS
Summary
DNA markers linked to the L4 gene that controls resistance to pepper mild mottle virus (PMMoV) in Capsicum spp. were screened. Among 516 arbitrary primers, only one primer (WA31) amplified a DNA fragment (WA31-1500) specific to the resistant group. A set of primers was designed based on the nucleotide sequence of WA31-1500. The newly synthesized primers amplified a single clear fragment (WA31-1500S) specific to the resistant individuals in the F2 population. This SCAR marker (WA31- 1500S) was shown to be linked to the L4 gene within a distance of 1.5 cM. WA31-1500S was amplified in 10 accessions possessing L4 gene, but not amplified in the other 11 susceptible accessions. The results strongly suggest that the WA31-1500S is closely linked to L4 gene, and should be useful as a selection marker in the breeding of PMMoV-resistant Capsicum.
Journal Citation
v.72(3):218-220, JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, JOURNAL OF THE
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