RAPD protocol for tagging of fertility restorer and male sterility genes in chilli (Capsicum annuum L.)

RAPD protocol for tagging of fertility restorer and male sterility genes in chilli (Capsicum annuum L.)

2002

A:PS

A stable nuclear male sterile line (MS-12), a stable cytoplasmically male sterile line (CCA-4261) and a strong restorer line (Pant C-1) were used to tag the male sterility (ms) and restorer (Rf) genes in chilli. A reproducible random amplified polymorphic DNA (RAPD) protocol was developed and the validity of reported RAPD markers associated with the Rf gene of chilli was tested in the restorer line Pant C-1. Preliminary results are presented on the screening for primers polymorphic between the pooled DNA of the male sterile and fertile plants of MAS-12 and CCA-4261, and maintainer F2 lines derived from the cross between CCA-4261 and Pant C-4. Among 22 primers tested, 10 generated scorable fragments in one or more of the four bulks. These fragments ranged between 4.2 kb to _5.6 kb. The primer OPP13 generated an unique fragment of about 1400 bp that was present in the DNA bulk of restorer plants and absent in the pooled DNA bulks of maintainer plants. It was concluded that the 1400 bp fragment may be linked to the Rf gene in Pant C-4.

v.29(2):101-105, VEGETABLE SCIENCE

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