Abstract
Some diploid and induced tetraploid plants of F1 Chinese cabbage (Brassica campestris subsp. pekinensis [B. pekinensis]) cv. Junsueng were crossed with pollen of diploid and induced tetraploids of F1 radish (Raphanus sativus var. major) cv. Taeback. On the 10th day after pollination, hybrid ovules, prior to their degeneration, were cultured in modified B5 medium supplemented with 0.1 mg NAA/litre and 0.1 mg 2,4-D/litre, and 591 hybrids were obtained. Some plants originating from 2n x 2n were treated with colchicine for chromosome doubling. An amphidiploid plant (2n=4x=38) was chosen for its high seed-bearing ability in self-pollination and used for androgenesis in anther culture. A breeding line derived from the anther culture was applied for microspore culture. One of the microspore-derived lines was very uniform and showed no mosaic symptoms when grown in the field. This amphidiploid, designated Brassicoraphanus, was finally selected and released as a new vegetable crop for its steady seed-bearing ability and its uniformity and suitability for field growing.