Short-duration colchicine treatment for in vitro chromosome doubling during ovule culture of Beta vulgaris L
1995
A:PS
Details
Title
Short-duration colchicine treatment for in vitro chromosome doubling during ovule culture of Beta vulgaris L
Publication Date
1995
Call Number
A:PS
Summary
Colchicine uptake into ovules of sugarbeet after 7 days of culture and its chromosome-doubling effect on ovule-derived plants were studied using high colchicine concentrations (0.4-6.0%) and short treatment duration (0-5 h) in the diploid lines TR015, XG006 and XW077. The best result (4.2 diploid plants produced per 100 ovules) was produced by treatment with 0.4% colchicine for 2.5 h. Both colchicine concentration and treatment time of ovules showed toxic effects on embryo formation, but it was stabilized at a low level with short exposure. In contrast, the chromosome-doubling effect was unchanged regardless of the colchicine concentrations used, but was highly affected by the duration of exposure. The greatest percentage of diploid plants (60%) was obtained after 3-5 h of uptake, which corresponds to only 31-39% of the total capacity for colchicine uptake in the ovules. Further uptake produced mainly toxic effects. Flow cytometric measurements of ploidy level in plantlets in vitro and of the same plants before flowering in soil were similar in about 80% of cases. Thus, flow cytometric selection of diploid plants in vitro may be an efficient tool.
Journal Citation
v.114(6):515-519, PLANT BREEDING
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles