Rapid preparation of DNA from filamentous fungi

Rapid preparation of DNA from filamentous fungi

1985

REP.TM-1295

We describe a general, simple and inexpensive method for the isolation of DNA from filamentous fungi. Starting from freeze-dried mycelium 0.1-0-15% by weight can be isolated as high molecular weight DNA suitable for restriction and ligation in 2 h. The preparation can be done in Eppendorf tubes and allows the processing of many samples in parallel. We have used the method with the basidiomycetes Phanerochaete chrysosporium, Coprinus cinereus and the ascomycete Asperyillus nidulans and others have used it with Trichoderrna reesei, Aspergillus niger and for the isolation of DNA from tomato plants.

v.1:17-20, LETTERS IN APPLIED MICROBIOLOGY

Research > Published Articles