Screening and evaluation of agronomically useful somaclonal variations in Japanese mint (Mentha arvensis)
1991
A:PS
Details
Title
Screening and evaluation of agronomically useful somaclonal variations in Japanese mint (Mentha arvensis)
Publication Date
1991
Call Number
A:PS
Summary
A procedure has been standardized for high frequency plant regeneration response from nodal explant cultures of M. arvensis var. piperascens. MS medium supplemented with IAA or NAA (0.5-2.0 mg/litre) alone, supported axillary shoot elongation while benzyladenine (2.0-3.0 mg/litre) + IAA (1.0 mg/litre) supported multiple shoot production. In vitro-derived shoots readily developed roots when cultured on NAA (1.0 mg/litre) fortified MS medium. Regenerated plantlets were successfully transferred to the greenhouse (90-95% survival rate) and ultimately to the field. Among 280 plants transferred to the field, a wide range of variation was observed for plant height (32.0-92.0 cm), leaf-stem weight ratio (0.53-2.32), herb yield (105.0-870.0g), oil content (0.32-1.10%) and oil yield (0.66-5.22 ml/plant). Variations were also recorded for 4 major constituents of the essential oil, i.e. menthol (65.2-94.77%), menthone (1.40-20.89%), isomenthone (0.96-5.14%) and menthyl acetate (0.75-8.52%). A positive correlation was found for oil yield with plant height and herb yield, whereas a negative correlation existed between herb yield and oil content. Based on the initial agronomic assessments on an individual plant basis, 27 somaclones were selected and further evaluated in a replicated plant to row trial with parent plant CIMAP/Hy-77 as standard. Somaclones Sc59 and Sc179, selected on the basis of higher herb yield in the initial screening, recorded 55.8% and 64.3% increase in oil yield over the control, respectively. Somaclones Sc93, Sc114, Sc121 and Sc124 that were selected for their better oil content exhibited 47.2%, 50.6% 57.5% and 48.2% increase in oil yield over the parent variety, respectively.
Journal Citation
v.53(3):183-191, EUPHYTICA
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles