Chemical composition of seed and electrophoretic pattern of seed storage protein of jute Corchorus olitorius and C. capsularis

1989

A:PS

Seeds of 7 cultivars of C. olitorius and 4 of C. capsularis were analysed for protein, oil and ash content. Seed weight in C. capsularis was almost twice that in C. olitorius. There were significant differences between cultivars in concentrations of protein, oil and ash. Oil, protein and ash contents were 11-16, 16-20 and 4.5-6.3%, respectively; mean contents in C. olitorius and C. capsularis were similar. The saponification number and iodine value for oil, estimated for 2 cultivars of each species, were 163-174 and 116-121, respectively. In the C. olitorius cv. TJ-40 and the C. capsularis cv. JRC-212, analysed in more detail, about 77% of seed N was in protein. On the basis of solubility in water and KCl, albumin and globulin accounted for 20 and 40%, respectively, of the seed protein. Three zones were observed following SDS-PAGE of seed protein extracted in SDS buffer. Zones 1, 2 and 3 consisted of polypeptides having MW around 60 000, 40 000 and 20 000, respectively. In zone 2, C. olitorius showed 2-3 major polypeptides of 38 000-41 000 MW, whereas C. capsularis had 5-6 polypeptides of 39 000-44 000 MW. The major polypeptides in the globulin fraction were linked by disulphide bonds. The globulin fraction, after reduction, consisted of 2 major polypeptides of MW around 40 000 and 20 000, which is similar to the legumin protein of legume seeds.

v.17(3):499-506, SEED SCIENCE AND TECHNOLOGY

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