Molecular taxonomy of Portuguese tronchuda cabbage and kale landraces using nuclear RFLPs

Molecular taxonomy of Portuguese tronchuda cabbage and kale landraces using nuclear RFLPs

1992

A:PS

RFLPs were used to study the genetic relationships of 31 Portuguese coles (Brassica oleracea) representing the different ecotypes or landraces grown in Portugal. Other cole crops such as Jersey kale (var. palmifolia), Chinese kales [B. alboglabra], common cabbages, broccolis and cauliflower, 2 nine-chromosome wild species (B. insularis and B. cretica) and one accession of turnip were also included to determine the evolutionary relationships of Portuguese coles to other related coles. Principal coordinates and cluster analyses were conducted using a package of computer programs and RFLP data from 55 nuclear DNA probes, detecting 291 polymorphic restriction fragments. The results showed that the 48 accessions clustered into five groups: (a) a dispersed group with turnip and the wild brassicas; (b) Chinese kales; (c) broccolis and cauliflower (d) a dispersed group including Algarve and Jersey kales, thousand head kale, cabbages and savoy cabbage; (e) a large and compact group containing all Portuguese tronchuda cabbages and kales. This last unique closely related group could be further divided into five subgroups corresponding to the major areas of cultivation. The phenetic groupings of Portuguese tronchuda cabbages and kales based on RFLP data corresponded more with their geographic origins (collecting sites) than with their morphological similarities. A mechanism involving geographic isolation and frequent intercrossing within local areas is proposed to explain genetic relationships among Portuguese tronchuda cabbages and kales.

v.58(3):221-229, EUPHYTICA

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