Purine metabolism in cotyledons and embryonic axes of black gram (Phaseolus mungo L.) seedlings

Purine metabolism in cotyledons and embryonic axes of black gram (Phaseolus mungo L.) seedlings

1983

REP.MB-2530

The metabolism of purine bases and nucleosides in cotyledons and embryonic axes of blackgram (Phaseolus mungo L.) was studied. A large portion of absorbed [8-14C]adenine, [8-14C]guanine and [8-14C]adenosine was salvaged in nucleotide and nucleic acid synthesis. Most of the radioactivity of [8-14C] hypoxanthine and [8-14C] inosine was incorporated into allantion and allantoic acid. Activity of adenine phosphoribosyltransferase in enzyme extracts was much higher than that of hypoxarnthine and guanine phosphoribosyltransferase(s). Apparent activity of adenosine kinase was higher than that of inosine kinase. NAD+ -dependent xanthine dehydrogenase was detected in both cotyledons and embryonic axex of the seedlings. The capacity of purine salvage was higher in 24 hr old cotyledons than 24 and 48 hr old embryonic axex. The reverse was observed concerning that of purine degradation. [AS]

v.15(8):1059-1065, INTERNATIONAL JOURNAL OF BIOCHEMISTRY

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