Characterization of a serologically distinct TSWV-like virus from impatiens

Characterization of a serologically distinct TSWV-like virus from impatiens

USDA/ARS United States

1991

p.53-59

SB608.T6H7

A TSWV-like virus(TSWV-I), which reacted poorly with TSWV antiserum, was isolated from the hybrid flower crop, New Guinea impatiens and later was found to be widespread in floral crop production in the United States. Three RNA's were isolated from purified virions of the TSWV-like isolate which comigrated with RNA's of known TSWV isolate. Hybridization between clones of TSWV-I S and M and the S and M RNA's of TSWV, respectively. Serological analysis demonstrated that while there was conservation of epitopes on the envelope proteins, G1 and G2, no serological relatedness was detected between the nucleocapsid proteins of TSWV and TSWV-I. Enveloped virions were relatively rare in TSWV-I infected tissue when assayed by immunosorbent electron microscopy or when tissues were examined in ultrathin section by electron microscopy. Paracrystalline arrays of filamentous structures were abundant in the TSWV-I infected tissue. These properties suggest that TSWV-I should be the type member of a second serogroup in the tomato spotted wilt virus group.[AS]

Virus-thrips-plant interactions of tomato spotted wilt virus; proceedings of a USDA workshop

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