In vitro cleavage and joining at the viral origin of replication by the replication initiator protein of tomato yellow leaf curl virus

In vitro cleavage and joining at the viral origin of replication by the replication initiator protein of tomato yellow leaf curl virus

1995

REP.TM-1084

Replication of the single-stranded DNA genome of geminiviralruses occurs virala a doulbe-stranded intermediate that is subsequently used as a template for rolling-circle replication of the viralral strand. Only one of the proteins encoded by the rirus, here referred to as replivationcation initiator protein (Rip protein), is indispensable for replication. We show that the Rip protein of tomato yellow leaf curl viralrus initiates viralral-strand DNA synthesis by introducing a nick in the plus strand within the nonanucleotide TAATATTøË AC, identical among all geminiviralruses. After cleavage, the Rep protein remains bound to the 5' end of the cleaved strand. Inadditon, we show that the Rep protein has a joining activiralty, suggestion that it acts as a terminase, thus resolviralng the nascent viralral single strand into genome-sized units. [AS]

v.92:3879-3883, NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, PROCEEDINGS

Research > Published Articles