HPLC-CLND application to quantitative nitrogen containing capsaicin compounds in red hot peppers

1994

REP.PP-0392

A novel high performance liquid chromatography-chemilumincscent nitrogen detector, HPLC-CLND, was recently described [1] .Capsaicin and dihydrocapsaicin in red hot peppers was quantitatively analyzed using a reverse-phase chromatography and a nitrogen-specific HPLC detection. Non-nitrogenous compounds in the sample matrix are transparent to the detector and therefore allow the chromatographer to focus on the separation of only the nitrogen containing compounds. A dual split HPLC-CLND and UV detection with a mobile phase flow rate at 1 mL/min is demonstrated. Detector linearity of the CLND under the split mobile phase flow conditions after the analytical HPLC column is obtained. The significance of this study is to provide a new and useful analytical approach for the analysis of these nitrogenous capsaicinoids which are responsible for the pungent flavor in certain spices and condiments. [AS]

v.34:367-379, DEVELOPMENTS IN FOOD SCIENCE

Research > Published Articles