Non-corrosive dye reagent for detection of reducing sugars in borate complex ion-exchange chromatography

Non-corrosive dye reagent for detection of reducing sugars in borate complex ion-exchange chromatography

1978

REP.G2034

The 2,2' -bicinchoninate reagent described previously for sugar detection in liquid chromatography with ethanol as eluent was adapted to an automated borate com plex ion-exchange chromatographic system, in which orcinol-sulphuric acid was used for detection. :The new aqueous reagents are non-corrosive and stable for several weeks; a better peak resolution can be achieved than with the viscous sulphuric acid reagent. Reaction times less than 1 min and reaction coils with I.D. down to 0.3 mm were used. Owing to the high baseline stability of the detection system with the new reagents, the analyzer can be run at higher sensitivity. The colour formation is linear over a detection range of more than two orders of magnitude. In routine work amounts of 0.1-30 ug of different sugars were determined.

v.156:197-204, JOURNAL OF CHROMATOGRAPHY

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