Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer
1988
REP.SB-1124
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Title
Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer
Author
Hinchee, M.A.W.
Connor-Ward, D.V.
Newell, C.A.
McDonnell, R.E.
Sato, S.J.
Gasser, C.S.
Fischhoff, D.A.
Re, D.B.
Fraley, R.T.
Horsch, R.B.
Connor-Ward, D.V.
Newell, C.A.
McDonnell, R.E.
Sato, S.J.
Gasser, C.S.
Fischhoff, D.A.
Re, D.B.
Fraley, R.T.
Horsch, R.B.
Publication Date
1988
Call Number
REP.SB-1124
Summary
Transgenic soybean plants have been produced using an Agrobacterium-mediated gene transfer system. This procedure relied on a regeneration protocol in which shoot organogenesis was induced on cotyledons of soybean genotypes selected for susceptibility to Agrobacterium. Cotyledon explants were inoculated with Agrobacterium tumefaciens pTiT37-SE harboring pMON9749 (conferring kanamycin resistance and beta-glucuronidase "GUS" activity) or pTiT37-SE:: pMON894 (conferring kanamycin resistance and glyphosate tolerance) and cultured on shoot induction medium containing kanamycin. Plantlets were tested for gene insertion 3-4 months post-inoculation. Approximately 6% of the shoots (8 plants to date) produced on the kanamycin-selected cotyledons were transgenic based on assays for GUS expression, kanamycin resistance or glyphosate tolerance. Progeny from two of these plants demonstrated co-segregation of kanamycin resistance and either GUS expression or glyphosate tolerance in a 3:1 ratio indicating a single insert inherited in a Mendelian fashion. [AS]
Journal Citation
v.6:915-922, BIOTECHNOLOGY
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles