In vitro culture of pepper (Capsicum annuum L.) anthers: high rate plant production from different genotypes by +35 C treatments

In vitro culture of pepper (Capsicum annuum L.) anthers: high rate plant production from different genotypes by +35 C treatments

1981

REP.PP-0034

New improvements are presented on the technique of in vitro culture of pepper anthers allowing high rates of haploid and diploid plant production from different cultivars. The most important factors of success are: 35 degree C temperature and darkness during the first days of in vitro culture on C medium complemented with 10-8 kinetin plus 10-8 2-4-D or 2 x 10-6 kinetin plus 10-7 2-4-D. The first growth substances association gives better results than the second one. Anther transfer, after 12 days culture on C medium, on a new culture medium with 10-7 kinetin and no 2-4 D (=R1). Plants directly arise on R1 medium after about 40 days. But an higher plant frequency is obtained if embryos are transferred on a new medium with 2 x 10-6 kinetin (=R2). With this technique, 5 to more 40 plants for 100 cultured anthers are obtained from large fruited cultivars (lines or F1 hybrid) and from F1 between large and small fruited cultivars. Among anther-derived plants high frequencies of diploids or haploid-diploid chimera were observed, sometimes more than 50 p. 100. [AS]

v.1(10):859-864, AGRONOMIE (PARIS)

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