Karyotype analysis of Chinese cabbage and cabbage by genome in situ hybridization

Karyotype analysis of Chinese cabbage and cabbage by genome in situ hybridization

2007

A:PS

To identify the different chromosomes between the A and C genomes of Brassica, labelled genomic DNAs of Chinese cabbage and cabbage were hybridized to their respective metaphase chromosomes. The non-uniform distribution of fluorescent-labelled probe DNAs was observed on all the chromosomes of both species. The signal patterns varied between the species and were related to the genome DNA probes. When Chinese cabbage genomic DNA was used as probe, the signals were detected almost exclusively in the pericentromeric regions and nucleolus organizer regions (NORs) of Chinese cabbage and cabbage, although the signal regions were larger in the former than in the latter. When cabbage genomic DNA was used as probe, the signals dispersed along the chromosome lengths of cabbage, and enhanced signal hands were shown in all pericentromeres and NORs. These patterns were observed only in the pericentromeres and NORs of Chinese cabbage chromosomes, and the signal intensity was weaker than when genomic DNA probes from Chinese cabbage were used. The karyotypes of Chinese cabbage and cabbage were analysed based on GISH signals. The results provided a molecular cytological basis for the identification of interspecific hybrids, their chromosomic components, and homology between the A and C genomes.

v.34(6):1459-1464, ACTA HORTICULTURAE SINICA

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