Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations
1991
REP.G1027
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Title
Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations
Publication Date
1991
Call Number
REP.G1027
Summary
We developed bulked segregant analysis as a method for rapidly identifying markers linked to any specific gene or genomic region was developed. Two bulked DNA samples are generated from a segregating population from a single cross. Each pool, or bulk, contains individuals that are identical for a particular trait or genomic region but arbitrary at all unlinked regions. The two bulks are therefore genetically dissimilar in the selected region but seemingly hetrozygous at all other regions. The two bulks can be made for any genomic region and from any segreating populaton. The bulks are screened for differences using restriction fragment length polymophism probes or random amplified polymorphic DNA primers. Bulked segregant analyis to identify three random amplified polymorphic DNA markers in lettuce linked to a gene for reistance downy mildew was used. Markers can be reliabley identifiedin a 25-centimorgan window on either side of the targeted locus. Bulked segregant analysis has several advantages over the user of near-isogenic lines to identifly markers in specific regions of the genome. Genetic walking will be possible by multiple rounds of bulked segregation analysis; each new pair of bulks will differ at a locus identified in the previous round of analysis. This approach will have widespread application both in those species where selfing is possible and in those that are obligatorily outbreeding. [AS]
Journal Citation
v.88:9828-9832, NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, PROCEEDINGS
Contact Information
harvest@worldveg.org
Record Appears in
Research > Published Articles