Detection of RAPD makers linked to gene lx1 in soybean

Detection of RAPD makers linked to gene lx1 in soybean

2004

A:PS

Near-isogenic lines of soyabean lipoxygenase, which contain genes Lox, lx1, lx2, lx3, lx1.3, and lx2.3 were used for polymorphic analysis by RAPD technique. A total of 520 10-mer-oligonucleotide primers were screened, and 13 primers showed polymorphism among near-isogenic lines. Six primers showed special polymorphic bands in lines lx1 and lx1.3. Primer S352 presented the stable results in which a 900 bp band was found in the lines lx1 and lx1.3, and primer S352900 was detected with F2 generation of cross 96P11 x Century-1, indicating that primer S352900 could be identified as an RAPD marker linked to gene lx1 in soyabeans. The linkage distance was 7.6 cM.

v.37(2):170-174, SCIENTIA AGRICULTURA SINICA

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