Alicyclic acid metabolism in plants. 12. Partial purification and some properties of shikimate kinase from Phaseolus mungo seedlings

Alicyclic acid metabolism in plants. 12. Partial purification and some properties of shikimate kinase from Phaseolus mungo seedlings

1979

A:PS

Shikimate kinase from P. mungo [Vigna mungo] seedlings was partially purified by DEAE-cellulose, hydroxyapatite and Sephacryl S-200 column chromatographies. The activity was completely inhibited by EDTA and the requirements for Mg2+ could be partially replaced by Mn2+, Ca2+, Co2+ and Cd2+. Sulphydryl inhibitor did not inhibit the enzyme activity. The apparent Km values for shikimic acid and ATP at pH 8.6 were 0.25 mM and 0.38 mM, resp. The activity appeared to be max. at pH 8.6-9.0. Shikimate-3-phosphate and ADP inhibited the activity slightly. Aromatic amino acids, quinic acid and dehydroquinic acid had no significant effect on the activity.

v.20(4):803-809, PLANT AND CELL PHYSIOLOGY

Research > Published Articles