Ploidy stability and in vitro chromosome doubling in gynogenic clones of onion (Allium cepa L.)

Ploidy stability and in vitro chromosome doubling in gynogenic clones of onion (Allium cepa L.)

1997

SB339.O5REP-0462

Ploidy stability in gynogenic clones of the long-day population De Mulhouse type Auxonne were assessed during micropropagation. In order to induce chromosome doubling in haploid forms, explants and plantlets of three clones were treated in vitro with colchicine (0.625-12.5 mM) and oryzalin (10-200 micro M) for 24 h (4 degrees C in the dark or 24 degrees C with a 16/8 h day/night photoperiod). Diploidization performed well (64%) during micropropagation on explants treated with 0.625 mM colchicine under 24 degrees C, 16/8 h day/night photoperiod conditions. Among treatments on individual plantlets, best results were obtained either with 2.5 mM colchicine (<65.7% of diploids) or 50 micro M oryzalin (<57.1% of diploids). Both chemicals induced mixoploids and affected plant regeneration, but oryzalin treatment produced better quality plants. Chromosome doubling in explants during the first cycle of micropropagation after gynogenesis appears to be a valuable and time-saving procedure to provide numerous doubled-haploid plants.

v.122:201-208, PLANT SCIENCE (INDIA)

Research > Published Articles