Record Details

Title:
A colorimetric method for assay of glutamate synthase in leaf tissue of Vigna mungo
Publication date:
1983
Summary:
Glutamine-2-oxo-glutarate aminotransferase (GOGAT) or glutamate synthase is generally assayed in terms of the disappearance of NAD(P)H from the assay medium. We describe a new technique which allows colorimetric measurement of the enzyme activity in cell-free extracts and whole tissue of Vigna mungo leaves in terms of disappearance of the substarate ( alpha-ketoglutarate). The technique is offered as an alternative, more convenient method for assay of GOGAT. This also eliminates the use of more sophisticated ultraviolet range instrumentation required for measurement of NAD(PH). [AS]
Call number:
REP.MB-2370
Journal citation:
v.61(1):290-294, CANADIAN JOURNAL OF BOTANY
Contact information:
harvest@worldveg.org

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 Record created 2017-10-01, last modified 2019-02-22


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