Isolation, characterization and phosphate-starvation inducible expression of potential Brassica napus PURPLE ACID PHOSPHATASE 17 (BnPAP17) gene family

Isolation, characterization and phosphate-starvation inducible expression of potential Brassica napus PURPLE ACID PHOSPHATASE 17 (BnPAP17) gene family

2008

A:PS

Three members of a Brassica napus PURPLE ACID PHOSPHATASE 17 (BnPAP 17) gene family were isolated. The full-length cDNAs of BnPAP17-1, BnPAP17-2 and BnPAP17-3 are 1277, 1356 and 1349 bp, with corresponding genomic sequences of 1466, 1594 and 1598 bp, respectively. The deduced 337-aa BnPAPI7-1, 333-aa BnPAP17-2 and 333-aa BnPAP17-3 proteins are all secretary low molecular weight (LMW) PAPs, containing a metallophos domain, 5-block conserved motifs and 7 metal-ligating residues. BnPAP 17-2 and BnPAP17-3 are highly similar to each other, but distinct from BnPAP17-1. Southern analysis suggests that these three genes comprise the entire BnPAP 17 gene family. They are all mainly transcribed in reproductive organs especially in bud. In vegetative organs, BnPAP 17-2 and BnPAP 17-3 are expressed in root, hypocotyl and stem, while BnPAP 17-1 expression is limited to root. In seedlings, these genes are all strongly induced by phosphate-starvation, and return to basal levels after phosphate resupply. Thus they are suggested to play important roles in reproductive development and adaptation to phosphorus deficiency.

v.49(3):199-213, BOTANICAL STUDIES

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