A new approach to chromosome doubling for haploid rice plants

A new approach to chromosome doubling for haploid rice plants

1989

REP.G1357

Nodal segments from 3 flowering haploids were excised, treated for 6, 8 or 10 h with 0.3% or 0.4% colchicine (dissolved in 2% DMSO) and then placed in vermiculite for rooting and sprouting. A combination of high colchicine concentration and long duration of treatment reduced the survival rate of treated segments, but increased the number of fertile plants that were produced. Pooled data showed that of the 842 segments used, 42.2% survived the treatment and sprouted, but only 31.9% were successfully established and grown to maturity. Among the 269 mature plants, 29.4% produced viable seeds, with an average of 146.2 seeds per diploidized plant.

v.77:149-151, THEORETICAL AND APPLIED GENETICS

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